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flow cytometry fixation permeabilization solution  (Becton Dickinson)

 
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    Becton Dickinson flow cytometry fixation permeabilization solution
    Flow Cytometry Fixation Permeabilization Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+fixation+permeabilization+solution/flow+cytometry+fixation+permeabilization+solution/pm37759541-53-37-40
    Average 90 stars, based on 1 article reviews
    flow cytometry fixation permeabilization solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    FACS:

    Article Title: Modeling of FAN1 -Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System.
    Article Snippet: They were treated with 20 nM Mitomycin C (MMC, #M0503, Sigma-Aldrich, St. Louis, MO, USA) on day 19 of differentiation for either 24 h or 48 h. .. Dissociated hiPSCs or kidney organoids using TrypLE Express were washed twice with FACS buffer (phosphate-buffered saline (PBS) containing 1% bovine serum albumin and 10 mM sodium azide), then permeabilized for 30 min using flow cytometry fixation and permeabilization solution (#554714; BD Biosciences, Torreyana Road, San Diego, CA, USA). .. Cells were stained with stage-specific embryonic antigens SSEA-4 (#813-70; 1:100, Santa Cruz Biotechnology, Dallas, TX, USA) or TRA-1–81 (#sc-21706; 1:100; Santa Cruz Biotechnology, Dallas, TX, USA) surface antibodies for 30 min. Intracellular staining for NANOG (#sc-293121;1:100, Santa Cruz Biotechnology, Dallas, TX, USA) was performed by means of sequential incubation with fixation and permeabilization solutions (#554722; BD Bioscience).

    Article Title: Prediction of diabetes mellitus after kidney transplantation using patient-specific induced pluripotent stem cells.
    Article Snippet: .. The cells were washed twice with FACS buffer (phosphate-buffered saline [PBS] containing 1% bovine serum albumin and 10-mM sodium azide), permeabilized for 30 minutes using flow cytometry fixation and permeabilization solution (554714; BD Biosciences), washed with wash buffer, stained with anti-OCT3/4 (60093AD.1; STEMCELL Technologies) and anti-insulin (565689; BD Biosciences) antibodies for 1 hour each, and then washed with FACS buffer. .. Samples were analyzed using a BD LSRFortessa cell analyzer (BD Biosciences).

    Saline:

    Article Title: Modeling of FAN1 -Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System.
    Article Snippet: They were treated with 20 nM Mitomycin C (MMC, #M0503, Sigma-Aldrich, St. Louis, MO, USA) on day 19 of differentiation for either 24 h or 48 h. .. Dissociated hiPSCs or kidney organoids using TrypLE Express were washed twice with FACS buffer (phosphate-buffered saline (PBS) containing 1% bovine serum albumin and 10 mM sodium azide), then permeabilized for 30 min using flow cytometry fixation and permeabilization solution (#554714; BD Biosciences, Torreyana Road, San Diego, CA, USA). .. Cells were stained with stage-specific embryonic antigens SSEA-4 (#813-70; 1:100, Santa Cruz Biotechnology, Dallas, TX, USA) or TRA-1–81 (#sc-21706; 1:100; Santa Cruz Biotechnology, Dallas, TX, USA) surface antibodies for 30 min. Intracellular staining for NANOG (#sc-293121;1:100, Santa Cruz Biotechnology, Dallas, TX, USA) was performed by means of sequential incubation with fixation and permeabilization solutions (#554722; BD Bioscience).

    Article Title: Prediction of diabetes mellitus after kidney transplantation using patient-specific induced pluripotent stem cells.
    Article Snippet: .. The cells were washed twice with FACS buffer (phosphate-buffered saline [PBS] containing 1% bovine serum albumin and 10-mM sodium azide), permeabilized for 30 minutes using flow cytometry fixation and permeabilization solution (554714; BD Biosciences), washed with wash buffer, stained with anti-OCT3/4 (60093AD.1; STEMCELL Technologies) and anti-insulin (565689; BD Biosciences) antibodies for 1 hour each, and then washed with FACS buffer. .. Samples were analyzed using a BD LSRFortessa cell analyzer (BD Biosciences).

    Flow Cytometry:

    Article Title: Modeling of FAN1 -Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System.
    Article Snippet: They were treated with 20 nM Mitomycin C (MMC, #M0503, Sigma-Aldrich, St. Louis, MO, USA) on day 19 of differentiation for either 24 h or 48 h. .. Dissociated hiPSCs or kidney organoids using TrypLE Express were washed twice with FACS buffer (phosphate-buffered saline (PBS) containing 1% bovine serum albumin and 10 mM sodium azide), then permeabilized for 30 min using flow cytometry fixation and permeabilization solution (#554714; BD Biosciences, Torreyana Road, San Diego, CA, USA). .. Cells were stained with stage-specific embryonic antigens SSEA-4 (#813-70; 1:100, Santa Cruz Biotechnology, Dallas, TX, USA) or TRA-1–81 (#sc-21706; 1:100; Santa Cruz Biotechnology, Dallas, TX, USA) surface antibodies for 30 min. Intracellular staining for NANOG (#sc-293121;1:100, Santa Cruz Biotechnology, Dallas, TX, USA) was performed by means of sequential incubation with fixation and permeabilization solutions (#554722; BD Bioscience).

    Article Title: Prediction of diabetes mellitus after kidney transplantation using patient-specific induced pluripotent stem cells.
    Article Snippet: .. The cells were washed twice with FACS buffer (phosphate-buffered saline [PBS] containing 1% bovine serum albumin and 10-mM sodium azide), permeabilized for 30 minutes using flow cytometry fixation and permeabilization solution (554714; BD Biosciences), washed with wash buffer, stained with anti-OCT3/4 (60093AD.1; STEMCELL Technologies) and anti-insulin (565689; BD Biosciences) antibodies for 1 hour each, and then washed with FACS buffer. .. Samples were analyzed using a BD LSRFortessa cell analyzer (BD Biosciences).

    Staining:

    Article Title: Prediction of diabetes mellitus after kidney transplantation using patient-specific induced pluripotent stem cells.
    Article Snippet: .. The cells were washed twice with FACS buffer (phosphate-buffered saline [PBS] containing 1% bovine serum albumin and 10-mM sodium azide), permeabilized for 30 minutes using flow cytometry fixation and permeabilization solution (554714; BD Biosciences), washed with wash buffer, stained with anti-OCT3/4 (60093AD.1; STEMCELL Technologies) and anti-insulin (565689; BD Biosciences) antibodies for 1 hour each, and then washed with FACS buffer. .. Samples were analyzed using a BD LSRFortessa cell analyzer (BD Biosciences).



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